c-Raf Kinase Assay Kit

Catalog #

79570

$600 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

c-Raf (Raf-1; proto-oncogen c-Raf) is a member of the Raf (rapidly accelerated fibrosarcoma) kinase family that plays an important role in regulating the MEK and ERK pathways. The c-Raf Kinase Assay Kit is designed for measuring c-Raf activity for screening and profiling applications.

Need us to run inhibitor screens or profile your compounds against c-Raf? Check out our Kinase Screening Services.

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Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40008	c-Raf	5 µg	-80°C	Avoid freeze/ thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
79569	5X Raf substrate	1 ml	-80°C
79696	96-well plate, white	1	Room Temp.