ALK1 (ACVRL1) Kinase Assay Kit
Catalog #
79549
$535
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Description
The ALK1 Kinase Assay Kit is designed to measure ALK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. The ALK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant ALK1 enzyme, ALK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.
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Synonyms
activin receptor-like kinase 1, ACVRL1
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The ALK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant ALK1 enzyme, ALK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Format
Catalog # | Reagent | Amount | Storage | |
40018 | ALK1 | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
79686 | ATP (500 µM) | 50 µl | -20°C | |
Casein (5 mg/ml) | 100 µl | -20°C | ||
79696 | 96-well plate, white | 1 | Room Temp. |
UniProt #
P37023
Background
ALK1, also known as activin receptor-like kinase 1 (ACVRL1) is a type I receptor from the TGF-β family of transmembrane serine/threonine kinases. Mutation of ALK1 is seen to play a role in pulmonary arterial hypertension (PAH), Osler-Weber-Rendu syndrome, and vascular smooth muscle cell development.
References
Cunha, Sara I., and Kristian Pietras. “ALK1 as an Emerging Target for Antiangiogenic Therapy of Cancer.” Blood 117(26): 6999–7006 (2011).