14-3-3 alpha/beta Protein, His-tag (Mouse)

Catalog #: 79024
Size: 20 μg
Species: Mouse
Host Species: Sf9 insect cells using baculovirus
MW: 32 kDa
Genbank #: NM_018753
Tag(s): N-terminal His tag
a.a: Full length
14-3-3 alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3 alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-alpha/beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-alpha/beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.
Mouse 14-3-3 alpha/beta Protein, GenBank Accession No. NM_018753, full length, with N-terminal His tag, expressed in Sf9 insect cells using baculovirus. MW = 32 kDa.
14-3-3 alpha/beta, Ywhab; 1300003C17Rik; 14-3-3b
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Storage / Stability:
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western blot
1. Yaffe, M B. et al: The structural basis for 14-3-3: phosphopeptide binding specificity. Cell 91: 961-971, 1997.

2. Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.

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