IL-2 Luciferase Reporter Jurkat Cell Line

Catalog #
60481
$6,875 *
Size: 2 vials
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Description

This recombinant Jurkat cell line was engineered for conditional expression of firefly luciferase. It contains a firefly luciferase gene under the control of a human Interleukin-2 (IL-2) promoter stably integrated into the genome.

The IL-2 Luciferase Reporter Jurkat cell line is suitable to monitor the activation of the IL-2 promoter. This reporter cell line has been functionally validated using various agonists such as phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin, an anti-human CD3 antibody, anti-human CD3/CD28 Dynabeads, and an anti-CD19/anti-CD3 bispecific antibody. It has also been validated in a co-culture assay with our TCR activator CHO cell line.

 

Figure 1. Illustration of IL-2 promoter region with representative transcription factor binding site.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of IL-2:IL-2R without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
itk, IL2
Product Info
Storage and Usage
Citations
Host Cell Line
Jurkat (clone E6-1), human T lymphoblast, suspension
Supplied As
Each vial contains ~2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Growth Medium 2B BPS Bioscience #79530

 

Materials Used in the Cellular Assays

Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Bovine Serum Albumin (BSA, protease free) Sigma #A4919
Phorbol 12-myristate 13-acetate (PMA) Fisher Scientific #BP685-1
Ionomycin Fisher Scientific #BP25271
Anti-CD3 Agonist Antibody BPS Bioscience #71274
TCR Activator - CHO Recombinant Cells  BPS Bioscience #60539
Dynabeads™ Human T-Activator CD3/CD28  Thermo Fisher #11131D
Raji Cells ATCC #CCL-86
Anti-CD19 x anti-CD3 bispecific antibody BPS Bioscience #100441
Cbl-b-IN-1 MedChemExpress #HY-136339
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
96-well tissue culture-treated white, clear-bottom plate   
Luminometer  
UniProt #
P14784
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Interleukin-2 (IL-2) is a key cytokine regulating the proliferation and differentiation of T cells into effector T cells. Gene expression of IL-2 is induced by activation of the TCR (T Cell Receptor) upon binding to MHC/peptide complexes. TCR activation results in the stimulation of transcription factors AP-1 (Activator protein 1), NFκB (Nuclear factor kappa-light-chain-enhancer of activated B cells) and NFAT (Nuclear factor of activated T-cells), which bind to canonical response elements in the promoter of IL-2. Co-stimulation of CD28 synergizes with the TCR to promote optimal IL-2 expression and secretion.

Induction of the luciferase reporter gene under the control of the IL-2 promoter is an excellent proxy for TCR activation in this Jurkat cell line.

References

1. Weaver JR et.al. (2007) Characterization of the sequence and architectural constraints of the regulatory and core regions of the human interleukin-2 promoter. Mol. Immunol. 44: 2813-2819.

2. Hughes CCW and Pober JS (1996) Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells. J. Biol. Chem. 271: 5369-5377.