IL-2 Luciferase Reporter Jurkat Cell Line
This recombinant Jurkat cell line was engineered for conditional expression of firefly luciferase. It contains a firefly luciferase gene under the control of a human Interleukin-2 (IL-2) promoter stably integrated into the genome.
The IL-2 Luciferase Reporter Jurkat cell line is suitable to monitor the activation of the IL-2 promoter. This reporter cell line has been functionally validated using various agonists such as phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin, an anti-human CD3 antibody, anti-human CD3/CD28 Dynabeads, and an anti-CD19/anti-CD3 bispecific antibody. It has also been validated in a co-culture assay with our TCR activator CHO cell line.
Figure 1. Illustration of IL-2 promoter region with representative transcription factor binding site.
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Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
Name | Ordering Information |
Thaw Medium 2 | BPS Bioscience #60184 |
Growth Medium 2B | BPS Bioscience #79530 |
Materials Used in the Cellular Assays
Name | Ordering Information |
Thaw Medium 2 | BPS Bioscience #60184 |
Bovine Serum Albumin (BSA, protease free) | Sigma #A4919 |
Phorbol 12-myristate 13-acetate (PMA) | Fisher Scientific #BP685-1 |
Ionomycin | Fisher Scientific #BP25271 |
Anti-CD3 Agonist Antibody | BPS Bioscience #71274 |
TCR Activator - CHO Recombinant Cells | BPS Bioscience #60539 |
Dynabeads™ Human T-Activator CD3/CD28 | Thermo Fisher #11131D |
Raji Cells | ATCC #CCL-86 |
Anti-CD19 x anti-CD3 bispecific antibody | BPS Bioscience #100441 |
Cbl-b-IN-1 | MedChemExpress #HY-136339 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
96-well tissue culture-treated white, clear-bottom plate | |
Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
Interleukin-2 (IL-2) is a key cytokine regulating the proliferation and differentiation of T cells into effector T cells. Gene expression of IL-2 is induced by activation of the TCR (T Cell Receptor) upon binding to MHC/peptide complexes. TCR activation results in the stimulation of transcription factors AP-1 (Activator protein 1), NFκB (Nuclear factor kappa-light-chain-enhancer of activated B cells) and NFAT (Nuclear factor of activated T-cells), which bind to canonical response elements in the promoter of IL-2. Co-stimulation of CD28 synergizes with the TCR to promote optimal IL-2 expression and secretion.
Induction of the luciferase reporter gene under the control of the IL-2 promoter is an excellent proxy for TCR activation in this Jurkat cell line.
1. Weaver JR et.al. (2007) Characterization of the sequence and architectural constraints of the regulatory and core regions of the human interleukin-2 promoter. Mol. Immunol. 44: 2813-2819.
2. Hughes CCW and Pober JS (1996) Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells. J. Biol. Chem. 271: 5369-5377.