IDO1 Fluorogenic Inhibitor Screening Assay Kit

Catalog #
72037
$780 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The IDO1 Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1). The IDO1 Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hourincubation at room temperature, the fluorescence solution is added and incubated at 37oC for four hours. Activity is measured by reading sample fluorescence at l=510 nm following excitation of the reaction product at l=400 nm.

Synonyms
Indoleamine 2,3-dioxygenase 1, IDO, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Supplied As
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 96 reactions.
Format

 

Catalog
Number

Component

Amount

Storage
71182 IDO1 His-Tag  40 μg  -80°C
Avoid
multiple
freeze/
thaw
cycles!       
73009 IDO1 Fluorogenic Reaction Solution 2 x 10 ml  -80°C
73002 IDO1 Assay Buffer  1 ml  -80°C
  Fluorescence Solution 2 x 1 ml -80°C
79685 Black 96 Well Assay-Plate  1 RT
  Plate Sealing Film 1  
UniProt #
P14902
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
References

Seegers, N., et al. J. Biomol. Screen. 2014. 19(9):1266-74.