CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72059

$1,070 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-γ signaling. The key to this kit is the high sensitivity of detection of biotin-labeled SIRP-γ through streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-γ is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against CD47:SIRP-γ[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, MER6, Signal regulatory protein-gamma, signal regulatory protein beta 2, and CD172g

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled SIRP-γ (CD172g), purified CD47, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format

Catalog #	Component	Amount	Storage
71236	SIRP-γ, Biotin-labeled	5 µg	-80°C	Avoid freeze/ thaw cycles!
71177	CD47	10 µg	-80°C
79742	Streptavidin-HRP	15 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79699	White 96-well microplate	1	+4°C

UniProt #

CD47: Q08772; SIRP-γ: Q9P1W8

Background

Signal-regulatory protein gamma (SIRP-λ), also known as CD172g, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-λ has been shown to be important for T cell trans-endothelial migration (TEM).

References

1. Stefanidakis, et al., Blood. 2008, 112(4):1280-1289

2. Sick, E., et al. Br J Pharmacol. 2012, 167(7):1415-1430.

Storage/Stability

Stable for 6 months from date of receipt when stored as directed.

Applications

This kit is useful for screening for inhibitors of SIRP-γ binding to CD47.

Shipping Temperature

-80°C (dry ice)

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy, Cell Surface Receptors

Regulatory Status

Research Use Only