CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit
The CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD40:CD40L signaling. This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD40L is coated on a 96-well plate. Next, CD40 is incubated with CD40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
- PBS (Phosphate buffered saline)
- Luminometer or microplate reader capable of reading chemiluminescence
- Rotating or rocker platform
Catalog # | Component | Amount | Storage | |
79102 | CD40, Biotin-labeled | 50 µg | -80 °C | Avoid multiple freeze/ thaw cycles |
71191 | CD40L-His | 10 µg | -80 °C | |
79311 | 3x Immuno Buffer 1 | 50 ml | -20 °C | |
79728 | Blocking Buffer 2 | 50 ml | +4 °C | |
79742 | Streptavidin-HRP | 10 µl | -20 °C | |
79670 | ELISA ECL substrate A(transparent bottle) | 6 ml | Room Temp. | |
ELISA ECL substrate B (brown bottle) |
6 ml | Room Temp. | ||
79699 | 96-well white microplate | 1 | +4 °C |
*The concentrations of CD40 and CD40L are lot-specific and will be indicated on the tubes containing the protein.