CD39 Inhibitor Screening Assay Kit

Catalog #

79278

$890 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD39 Inhibitor Screening Assay Kit is a colorimetric assay designed to measure the activity of CD39 for screening and profiling applications. The kit comes in a convenient 96-well format with enough purified CD39 enzyme, ATP, CD39 assay buffer, and colorimetric detection reagent for 100 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.

Need us to run inhibitor screens or profile your compounds against CD39? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen

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Product Data Gallery

Ihibition of CD39 enzyme activity by POM-1.

Product Info

Storage and Usage

Citations

Assay Kit Format

Colorimetric

Supplied As

The kit comes in a convenient 96-well format with enough purified CD39 enzyme, ATP, CD39 assay buffer, and colorimetric detection reagent for 100 enzyme reactions.

Materials Required But Not Supplied

UV/Vis spectrophotometer microplate reader capable of reading λ=630 nm
Adjustable micropipettor and sterile tips

Format

Catalog #	Reagent	Amount	Storage
71284	CD39, His-Tag*	1 µg	-80°C
79279	4x CD39 Assay Buffer	3 ml	-20°C
	35 mM ATP	15 µl	-20°C
	POM-1, MW=2986.01 g/mol**	1.5 mg	-20°C
74001	Colorimetric Detection Reagent***	10 ml	4°C
79963	Transparent 96-well microtiter plate	1	Room Temp

*The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
**CD39 inhibitor POM-1 is provided as a control for CD39 inhibition.
***Colorimetric Detection Reagent is used to measure the free phosphate generated in the reaction catalyzed by CD39. Any other source of inorganic phosphate will interfere with the assay.

UniProt #

P49961

Background

B cell activation marker Cluster of Differentiation 39 (CD39), also known as ecto-apyrase, ATP diphosphohydrolase, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), hydrolyzes extracellular nucleotide tri- and diphosphates into AMP in the presence of Ca²⁺ and Mg²⁺. It is an important enzyme in many biological processes, including the modulation of neural cell activities, prevention of intravascular thrombosis, and regulation of immune responses. The role of regulatory T cells (Treg) links closely to CD39/CD79, and a dysfunction in this pathway can lead to cancer progression. The use of CD39 inhibitors can prove advantageous for the treatment of pathologies where CD39/CD73 pathways play a role.

References

1. Wang, T.-F., et al., J. Biol. Chem. 273 (38), 24814-21 (1998)

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and High Throughput Screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only