CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit

Catalog #: 72025
Size: 96 reactions
CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4- 1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.

The CD137:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CD137 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

4-1BB, Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA, 4-1BB ligand, 4-1BBL, tumor necrosis factor ligand superfamily member 9, TNFSF9, activity assay kit, Strep HRP, streptavidin
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.


Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
This kit is useful for screening for inhibitors of CD137 binding to CD137L.
1. Drenkard, D., et al., FASEB J. 2007; 21: 456-463
2. Kwon, B., et al., Immune Netw. 2009; 9(3): 84-89.
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
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