CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit
The CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. This assay takes advantage of the highly sensitive detection of biotin-labeled CD137 by streptavidin-HRP. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
- PBS (Phosphate buffered saline)
- Luminometer or microplate reader capable of reading chemiluminescence
- Rotating or rocker platform
Catalog Number |
Component |
Amount |
Storage |
71171 | CD137 (4-1BB), His-tagged, Biotin-labeled | 2 µg | -80 °C |
71189 | CD137L (4-1BB ligand), His tagged | 10 µg | -80 °C |
79742 | Streptavidin-HRP | 10 µl | +4°C |
79311 | 3x Immuno Buffer 1 | 50 ml | -20 °C |
79728 | Blocking Buffer 2 | 50 ml | +4 °C |
79670 | ELISA ECL substrate A (transparent bottle) |
6 ml | Room Temp. |
ELISA ECL substrate B (brown bottle) |
6 ml | Room Temp. | |
79699 | 96-well white microplate | 1 | Room Temp. |
CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.
1. Drenkard, D., et al., FASEB J. 2007; 21: 456-463
2. Kwon, B., et al., Immune Netw. 2009; 9(3): 84-89.