BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72008
Size: 96 reactions
The binding of BTLA to the HVEM receptor (herpesvirus entry mediator, also known as TNFRSF14) triggers cell signaling pathways involved in the negative regulation of T cells. BTLA:HVEM interaction plays a key role in the regulation of inflammatory, autoimmune, and antitumor responses, and is an important target for drug discovery.

The BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BTLA:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BTLA is coated on a 96-well plate. Next, HVEM is incubated with BTLA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

CD272, CD270, TNFRSF14, ATAR, HVEA, LIGHTR, TR2 , activity assay kit, Strep HRP, streptavidin
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled HVEM, purified BTLA, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt.


Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
This kit is useful for screening inhibitors of HVEM binding to BTLA

1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167.
2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.

Avoid freeze/thaw cycles.
Scientific Category:
Cell Surface Receptor/Single-pass Transmembrane Protein
Product Type:
Assay Kit
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