IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line

Catalog #

82591

$12,500 *

Size: 2 vials

Qty

Bulk/Custom

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Description

IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line is an engineered HEK293 cell line expressing firefly luciferase under the control of STAT3 response element, and human IL-23 receptor complex (IL12Rβ1 NM_005535.3 and IL23R NM_144701.3). With this cell line, IL-23 activity can be monitored by measuring luciferase activity.

The functionality of the IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line was validated in dose-response assays using a recombinant human interleukin 23 (human IL-23; p19 + p40), inhibition assays using the anti-IL-23 antibodies Ustekinumab and Guselkumab, as well as a Janus Kinase inhibitor, Ruxolitinib.

Figure 1. Illustration of the mechanism of action in the IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Interested in screening and profiling IL-23 cells using your cell models without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

IL-23 receptor; IL-23R, Interleukin-23 receptor, IL23R

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Product Data Gallery

Analysis of the expression of IL23R and IL12Rβ1 in the IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line by flow cytometry.

Dose response curve of IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line to recombinant human IL-23 in a 96-well assay format.

Dose response curve of IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line to recombinant mouse IL-23 in a 96-well assay format.

Dose response curve of IL-23 Responsive STAT3Luciferase Reporter HEK293 Cell Line to recombinant human IL-23 in a 384-well assay format.

Inhibition of IL-23 induced reporter activity by Guselkumab in IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Inhibition of IL-23 induced reporter activity by Ustekinumab in IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Inhibition of IL-23 induced reporter activity by Anti-p40 Antibody (#102108) in IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Inhibition of IL-23 induced reporter activity by Anti-IL23α Neutralizing Antibody in IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Inhibition of IL-23 induced reporter activity by Ruxolitinib in IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1U	BPS Bioscience #78548
Cell Freezing Medium	BPS Bioscience #79796

Materials Required for Cellular Assay

Name	Ordering Information
IL-23, His-Tag recombinant	Peprotech #200-23
Anti-IL-12 p40 Neutralizing Antibody	BPS Bioscience #102108
Anti-IL23α Neutralizing Antibody	BPS Bioscience #101453
Guselkumab	SelleckChem #A2438
Ruxolitinib	Cayman #11609
Recombinant Mouse IL-23 Protein	Sino Biological #CT028-M08H
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

UniProt #

Q5VWK5

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

IL-23, a heterodimeric complex composed of p40 and p19, is a proinflammatory cytokine produced by activated macrophages and dendritic cells. This cytokine plays a crucial role in immune responses by promoting the production of interferon-gamma (IFN-γ) by helper T cells and supporting the proliferation of IL-17-secreting CD4⁺ T cells. Due to its involvement in various autoimmune diseases, including inflammatory bowel disease (IBD), colitis, psoriasis, and arthritis, significant efforts are underway to develop reagents that can antagonize the IL-23 signaling pathway.

References

Yang K., et al., 2021 Am J Clin Dermatol. 22(2): 173-192.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1U (BPS Bioscience #78548): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 0.5 µg/ml of Puromycin and 100 µg/ml of Hygromycin B.

Media Required for Functional Cellular Assay
Assay Medium: Thaw Medium 1

Applications

Screen and characterize anti-IL23 antibodies

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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IL-23 Responsive STAT3 Luciferase Reporter HEK293 Cell Line

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