Human Interleukin-1 alpha Recombinant

Catalog #

90169-A

$130 *

Size: 2 µg

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Description

Recombinant Interleukin-1a is a disulfide-linked monomer protein consisting of 160 amino acid residues and migrates as an approximately 18 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-1 alpha mature chain was expressed in E. coli.

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Synonyms

IL-1A, IL-1alpha, Interleukin-1 alpha, Hematopoietin-1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS, pH 7.0.

18 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

113–271

Biological Activity

The ED₅₀ was determined by the dose-dependent stimulation of murine D10S cells is ≤ 0.002 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁹ units/mg.

Genbank #

P01583

UniProt #

P01583

Background

Monocytes are the main source of secreted IL-1. They express predominantly IL-1beta while human keratinocytes express large amounts of IL-1alpha. Murine macrophages display a transition from IL-1beta to IL-1alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL-1, IL-1alpha and IL-1beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL-1alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1beta but not the IL-1alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

References

1. Invest. Ophthalmol. Vis. Sci., Apr 2009, 50: 545.
2. J. Immunol., Dec 2008, 181: 8194 - 8198.
3. Invest. Ophthalmol. Vis. Sci., Apr 2008, 49: 1657.

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Human Interleukin-1 alpha Recombinant

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