Hrd1, SUMO-His-tags Recombinant

Catalog #
81051
$410 *
Size: 25 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Cytoplasmic region of human Hrd1 recombinant protein (Genbank Accession No. ), a.a. 236-17, with N-terminal SUMO-tag, expressed in E. coli.

Synonyms
E3 ubiquitin-protein ligase synoviolin, HRD1, KIAA1810, MGC40372, Synovial apoptosis inhibitor 1
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥85% by SDS-PAGE
Format
Aqueous buffer solution
Formulation
PBS, 10% glycerol
MW
39.3 kDa (without SUMO tag)
Amino Acids
236–617
Biological Activity
Typical enzyme concentration of 20-1000 nM is used for in vitro conjugation depending on assay conditions.
Genbank #
Q86TM6.2
UniProt #
Q08109
Tag(s)
N-terminal SUMO-His-tags
Background
Hrd1 is a RING domain E3 ligase that is involved in the conjugation of ubiquitin to target substrates. Hrd1 has been demonstrated to function with the E2 enzymes UBE2D3 (UbcH5c) and UBE2G2 in vitro. Hrd1 is an ER-associated ligase involved in ERAD and has been linked to rheumatoid arthritis. It is present in human rheumatoid synovial cells and found to be a causative factor for arthropathy in in vivo studies. Hrd1 is also found in brain neurons and its proper regulation may be linked to neurodegenerative diseases. This construct is a Hrd1 N-terminal deletion containing residues 246-617 consisting of the complete C-terminal cytoplasmic portion. The N-terminal transmembrane spanning portion has been deleted. This Hrd1 N-terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains.
References
1. Kaneko, M., et al. Molecular approaches to the treatment, prophylaxis, and diagnosis of Alzheimer's disease: possible involvement of HRD1, a novel molecule related to endoplasmic reticulum stress, in Alzheimer's disease. J. Pharmacol. Sci, 2012. 118(3):325-30.
2. Yagishita, N., et al. Synoviolin, protein folding and the maintenance of joint homeostasis, Nat. Clin. Prac. Rheumatol, 2008. 4(2):91-7.
3. Yamasaki, S., et al. The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apoptosis and p53 pathway. Cell Cycle 6(11):1319-23.