TetR HEK293 Cell Line

Catalog #
71227
$1,250 *
Size: 2 vials
Qty
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Description

The TetR HEK293 Cell Line is a HEK293 cell line expressing the repressor of the tetracycline resistance element (TetR), creating an inducible system based on the presence of Tetracycline or Doxycycline in the cell culture media. These cells can be transfected with a target of interest under the control of a strong promoter and the Tetracycline Operon. In the absence of tetracycline or doxycycline, TetR binds to the operon and represses transcription. When exposed to tetracycline or doxycycline, TetR loses affinity for the tetracycline resistance element expression of the target of interest occurs.

This cell line has been validated by stimulation with doxycycline.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Product Info
Storage and Usage
Citations
Host Cell Line
HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Species
Human
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied
Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1H BPS Bioscience #79546
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

TetR, or Tet repressor proteins play a role in development of antibiotic resistance to tetracycline (Tc). Tc are a family of antibiotics that kills bacteria by interfering with protein synthesis. Resistance to Tc occurs by expression of Tc resistance genes, which are regulated by TetR. In addition to the study of mechanisms of antibiotic resistance, the use of TetR has been used as a mode to regulate promoter expression, and thus create inducible cell models. These inducible cell models have been crucial for the understanding of cellular pathways.

References

On the use of the T-Rex tetracycline-inducible gene expression system in vivo. Dobrovolsky VN1, Heflich RH. 2007. Biotechnol Bioeng. 98(3):719-23.