HSP90α (N-terminal) Assay Kit

Catalog #

50298

$1,015 *

Size: 384 reactions

Qty

Bulk/Custom

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Description

The HSP90α (N-terminal) Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

This product has been cited 11 times.

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Synonyms

HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α

Product Info

Storage and Usage

Citations11

Assay Kit Format

Fluorescence Polarization

Supplied As

The HSP90α inhibitor screening assay kit comes in a convenient 384-well format, with purified HSP90α enzyme, fluorescently labeled geldanamycin and HSP90 assay buffer for 384 enzyme reactions.

Format

Catalog #	Component	Amount	Storage
50290	Hsp90α recombinant enzyme	140 µg	-80°C	Avoid freeze/ thaw cycles!
50312	FITC-labeled geldanamycin (2.5 µM)	2 x 30 µl	-80°C
50311	5x Hsp90 Assay Buffer1	2 x 4 ml	-20°C
79961	Low binding black microtiter 384-well plate	1	Room Temp

UniProt #

P07900

Background

Hsp90α is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90α function has been shown to play a role in tumorigenesis and disease progression.

References

1. Kim, J., et al., J Biomol Screening. 2004, 9(5): 375-381.
2. Howes, R., et al., Anal Biochem. 2006, 350:202-213.

Storage/Stability

Stable for at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.  

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Heat Shock Proteins

Regulatory Status

Research Use Only

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HSP90α (N-terminal) Assay Kit

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