GLS2 Inhibitor Screening Assay Kit

Catalog #
79925
$780 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The GLS2 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS2 for screening and profiling applications.

Synonyms
Liver-type glutaminase assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Supplied As
The GLS2 assay kit comes in a convenient 96-well format, with purified GLS2, its substrates, the Coupling reagent, and buffer for 100 enzyme reactions.
Materials Required But Not Supplied

0.5 M dithiothreitol (DTT) in aqueous solution
Adjustable micropipettor and sterile tips
Fluorescent microplate reader capable of reading λexcitation = 340 nm; λemission = 460 nm

Format
Catalog
Number

Component

Amount

Storage
71242 GLS2, His-tag 30 µg -80°C

Avoid

multiple
freeze/
thaw
cycles
L-Glutamine (100 mM) 100 µl -20°C
NAD+ (20 mM) 1 ml -20°C
Coupling reagent 10 µl -20°C
4X GLS assay buffer 2.5 ml -20°C
79685 96-well black microplate 1 Room
Temp
UniProt #
Q9UI32
Background

Liver-type glutaminase (GLS2) is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of L-glutamine to L-glutamate and ammonia. GLS2 is related to tumor progression and cancer, including breast and hepatocellular carcinoma.

References
  1. Xiang, Lisha, et al. (2013) "Knock-down of glutaminase 2 expression decreases glutathione, NADH, and sensitizes cervical cancer to ionizing radiation." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1833.12: 2996-3005.
  2. Zhang, Jianbin, et al. (2013) "Epigenetic silencing of glutaminase 2 in human liver and colon cancers." BMC cancer 13.1: 601-609.