GLS2 Inhibitor Screening Assay Kit
Catalog #
79925
$780
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Description
The GLS2 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS2 for screening and profiling applications.
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Synonyms
Liver-type glutaminase assay kit
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Supplied As
The GLS2 assay kit comes in a convenient 96-well format, with purified GLS2, its substrates, the Coupling reagent, and buffer for 100 enzyme reactions.
Materials Required But Not Supplied
0.5 M dithiothreitol (DTT) in aqueous solution
Adjustable micropipettor and sterile tips
Fluorescent microplate reader capable of reading λexcitation = 340 nm; λemission = 460 nm
Format
Catalog Number |
Component |
Amount |
Storage |
|
71242 | GLS2, His-tag | 30 µg | -80°C | Avoid multiple freeze/ thaw cycles |
L-Glutamine (100 mM) | 100 µl | -20°C | ||
NAD+ (20 mM) | 1 ml | -20°C | ||
Coupling reagent | 10 µl | -20°C | ||
4X GLS assay buffer | 2.5 ml | -20°C | ||
79685 | 96-well black microplate | 1 | Room Temp |
UniProt #
Q9UI32
Background
Liver-type glutaminase (GLS2) is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of L-glutamine to L-glutamate and ammonia. GLS2 is related to tumor progression and cancer, including breast and hepatocellular carcinoma.
References
- Xiang, Lisha, et al. (2013) "Knock-down of glutaminase 2 expression decreases glutathione, NADH, and sensitizes cervical cancer to ionizing radiation." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1833.12: 2996-3005.
- Zhang, Jianbin, et al. (2013) "Epigenetic silencing of glutaminase 2 in human liver and colon cancers." BMC cancer 13.1: 601-609.