GLS2, His-tag Recombinant

Catalog #

71242

$510 *

Size: 20 µg

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Bulk/Custom

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Description

Recombinant human GLS2 (glutaminase 2), encompassing amino acids 15-602 (end). This construct contains an N-terminal His-tag (6xHis). The protein was affinity purified.

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Synonyms

glutaminase liver isoform (liver, mitochondria), L-glutaminase, and L-glutaminase amidohydrolase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

GLS2 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

GLS2 (His-15-602(end))

Host Species/Expression System

E. coli

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, variable Imidazole.

66 kDa

Amino Acids

15-602(end)

Genbank #

NM_013267

UniProt #

Q9UI32

Tag(s)

N-terminal His-tag

References

1. Gross, M.I. et al., Mol. Cancer Ther. 2014; 13: 890.
2. Szeliga, M. et al., Tumor. Biol. 2014; 35: 1855-1862.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to BPS catalog #79596 with a modified incubation time of 10 minutes and GLS2 titrated from 300 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only

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Datasheets/Protocols

71242 Lot 200109-1

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