GLS1, His-tag Recombinant

Catalog #

71102

$555 *

Size: 20 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human glutaminase transcript variant 1 (GLS1), also known as GAC, GAM and KGA, GenBank Accession No. NM_014905, a.a. 17-669(end), with N-terminal His-tag, expressed in an E. coli cell expression system. MW=73 kDa.

●

Synonyms

glutaminase, glutaminase transcript variant 1, GLS1, GAC, GAM, KGA, GLS

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

GLS1 inhibition curve using a fluorogenic assay (#79596). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

80%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.

73 kDa

Amino Acids

17–669(end)

Specific Activity

≥80%

Genbank #

NM_014905

UniProt #

O94925

Tag(s)

N-terminal His-tag

Background

GLS1 is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. GLS1 is primarily expressed in the brain and kidney where it catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

References

1. Van den Heuvel, A.P., et al., Cancer Biol. Ther. 2012;13(12):1185-1194.
2. Thangavelu, K., et al., Proc. Natl. Acad. Sci. USA 2012;109(2):7705-7710.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to the GLS1 Inhibitor Screening Assay Kit (BPS Bioscience, #79596) with GLS1 titrated from 40- 0 ng.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Metabolic Enzymes-Transferase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

71102 Lot 200423-A

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

GLS1, His-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY