SIRT2 (Sirtuin2) Fluorogenic Assay Kit

Catalog #

50087

$700 *

Size: 32 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic SIRT2 Assay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements*.The kit includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against SIRT2 (Sirtuin2)? Check out our Deacetylase/Sirtuin Screening Services.

This product has been cited 4 times.

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Synonyms

sirt2, sirtuin 2

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Product Data Gallery

Product Info

Storage and Usage

Citations4

Assay Kit Format

Fluorogenic

Supplied As

Kit comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements.

Format

UniProt #

Q8IXJ6

References

1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only