The Fluorogenic Prolyl OligoPeptidase (POP) Assay Kit is a complete assay system designed to measure activity of the purified POP enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive materials and chromatography in traditional assays. Purified human recombinant POP is included in the kit as a positive control. Using this kit, only one simple step, in which the fluorometric substrate is incubated with purified POP, is needed to analyze the POP activity level. The resulting fluorescent product can then be easily measured with a microtiter-plate fluorimeter.
Need us to run inhibitor screens or profile your compounds against POP? Check out our Protease Screening Services.
Supplied in a convenient 96-well format, with purified POP enzyme, substrate, and assay buffer for 100 enzymereactions.
Format
UniProt #
P48147
Background
Prolyl oligopeptidase (POP), also known as prolyl endopeptidase
(PREP), is a cytosolic serine peptidase involved in the maturation and degradation of
peptide hormones and neuropeptides.
References
1. Myohanen T.T. et al., Neurochem. Res. 32 (8), 1365-1374 (2007). 2. Garcia-Horsman J.A. et al., Scand. J. Gastroenterol. 42 (5), 562-571 (2007).
Storage/Stability
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Notes
The AMC standard is included so the researcher can quatitatively determine the
specific activity of the enzyme using the AMC standard as a measure of how much
AMC substrate was cleaved to release free AMC.
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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