DNMT Universal Chemiluminescent Assay Kit
The DNMT Universal Assay Kit is designed to measure DNMT activity for screening and profiling applications. The key to the DNMT Universal Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
This product has been cited 3 times.
Catalog # |
Name |
Amount |
Storage |
|
10 µg |
-80°C |
(Avoid freeze/ thaw cycles!) |
||
10 µg |
-80°C |
|||
10 µg |
-80°C |
|||
250 µl |
-80°C |
|||
|
Anti-5-methylcytosine antibody |
25 µl |
-80°C |
|
52130H |
Secondary HRP-labeled antibody 1 |
10 µl |
-80°C |
|
5 ml |
-20°C |
|||
50 ml |
+4°C |
|||
|
HRP chemiluminescent substrate (2 components) |
6 ml each |
+4°C |
|
|
8-well strip plate precoated with DNMT substrate |
1 |
+4°C |
*Add 10 μl of 0.5 M DTT before use
Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.