DNMT Universal Chemiluminescent Assay Kit

Catalog #
52035
$1,425 *
Size: 96 reactions
Qty
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Description

The DNMT Universal Assay Kit is designed to measure DNMT activity for screening and profiling applications. The key to the DNMT Universal Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

This product has been cited 3 times.

Product Info
Storage and Usage
Citations3
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT1, DNMT3A/3L and DNMT3B/3L for 100 reactions.
Format

Catalog #

 Name

Amount

Storage

51101

DNMT1

10 µg

-80°C

 (Avoid freeze/ thaw cycles!)

51106

DNMT3A/3L Complex

10 µg

-80°C

51109

DNMT3B/3L Complex

10 µg

-80°C

52120

400 µM S-adenosylmethionine

250 µl

-80°C

 

Anti-5-methylcytosine antibody

25 µl

-80°C

52130H

Secondary HRP-labeled antibody 1

10 µl

-80°C

52201

4x DNMT assay buffer 2*

5 ml

-20°C

52100

Blocking buffer 4

50 ml

+4°C

 

HRP chemiluminescent substrate (2 components)

6 ml each

+4°C

 

8-well strip plate precoated with DNMT substrate

1

+4°C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*Add 10 μl of 0.5 M DTT before use

UniProt #
P26358
References

Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.