DEP1(PTPRJ), GST-tag Recombinant

Catalog #

30050

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), also known as PTPRJ, PTP-eta and CD148 (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system.

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Synonyms

DEP1, density-enhanced phosphatase-1, PTP-eta, CD148, PTPRJ

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

58.9 kDa

Amino Acids

1024–1306

Specific Activity

268 nmole/min/µg

Genbank #

NM_002843

UniProt #

Q12913

Tag(s)

N-terminal GST-tag

Background

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Positive regulator of platelet activation and thrombosis. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation through KDR dephosphorylation. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration through dephosphorylation of PDGFR. Negative regulator of EGFR signaling pathway via EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.

References

1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12.
2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.

Storage/Stability

>12 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30&Deg;C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε _pNPP= 18,000 M^-1cm^-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Receptor

Regulatory Status

Research Use Only