CD40 - HEK293 Cell Line

Catalog #: 71257
Size: 2 vials
CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, other cell types such as monocytes, basophils, dendritic cells, endothelial cells, and epithelial cells have been found to express CD40. High levels of CD40 have also been detected in numerous human cancers, including HCT116, a colon cancer epithelial cell line. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC), promote anti-tumor T-cell responses, and to foster cytotoxic myeloid cells, suggesting a potential mechanism to control cancer in the absence of T-cell immunity.
Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot.
Host Cell line:
Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Thermo Fisher, Cat. #26140079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1F (BPS Bioscience #79540): Thaw Medium 1, 100 µg/ml Hygromycin B (Thermo Fisher, Cat. #10687010).

Each vial contains ~2 x 10^6 cells in 1mL of 10% DMSO in FBS.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Since HEK293 cell line does not express endogenous CD40, the CD40 HEK293 Cell Line is ideal to monitor CD40-specific signaling responses, such as NF-κB activation in response to potential CD40 agonistic monoclonal antibodies.
1. Diaconu I. (2012) Immune Response is an Important Aspect of the Anti-tumor Effect Produced by a CD40L-Encoding Oncolytic Adenovirus. Cancer Res. 72: 2327.

2. Yacoub D (2013) CD154 is Released from T-cells by a Disintegrin and Metalloproteinase Domain-containing Protein 10 (ADAM10) and ADAM17 in a CD40 Protein-dependent Manner. J. Biol. Chem. 288: 36083.


License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

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Product Type:
Cell Line
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