CD27/NF-kB Reporter-Jurkat Recombinant Cell Line

Catalog #: 79509
Size: 2 vials
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27 signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the induction of long-term memory. The CD27/CD70 pathway is a key target for the development of treatments for cancer and inflammatory diseases.
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NF-kB response elements with constitutive expression of human CD27 (also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, Genbank Accession #BC012160)
Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, T14, cd27 cell line, cd-27
Supplied As:
Each vial contains 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
Mycoplasma Testing:

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.

Growth Media:

Thaw Medium 2 (BPS #60184): RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

Growth Medium 2C (BPS #79592): Thaw Medium 2 (BPS Bioscience #60184), 1 mg/ml of Geneticin (Life Technologies #11811031), and 100 μg/ml of Hygromycin B (Hyclone #SV30070.01)

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Store in liquid nitrogen immediately upon receipt

•Screen for agonists or antagonists of CD27-CD70 signaling in a physiologically relevant cellular context

•Characterize T cell-mediated immune responses of CD27 and its interaction with CD70

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

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