CD155 / TCR Activator - CHO Recombinant Cell line

Catalog #: 60548
Size: 2 vials
TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.
Recombinant CHO-K1 cells constitutively expressing human CD155 (Poliovirus receptor, Nectin-like protein 5, GenBank accession # NM_006505) and an engineered T cell receptor (TCR) activator.
Materials Required But Not Provided:

Materials Required for Cell Culture

• Thaw Medium 3 (BPS Bioscience #60186): Ham’s F-12 medium (Hyclone, #SH30526.01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)

• Growth Medium 3C (BPS Bioscience, #79537)

Materials Required for Cellular Assay 

TIGIT/NFAT Reporter - Jurkat Cell Line (BPS Bioscience #60538)

Assay Medium 2C (BPS Bioscience #79623)

• Thaw Medium 3 (BPS Bioscience #60186): Ham’s F-12 medium (Hyclone, #SH30526.01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)

Anti-TIGIT neutralizing antibody (BPS Bioscience #71340)

• 96-well tissue culture-treated white clear-bottom assay plate 

One-Step luciferase assay system (BPS Bioscience #60690) or other luciferase reagents for measuring firefly luciferase activity

• Luminometer 

Poliovirus receptor, Nectin-like protein 5, CD155-His
Host Cell line:
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorĀ®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone, #SH30526.01) supplemented with 10% FBS (Life technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 3C (BPS Bioscience, #79537): Thaw Medium 3 (BPS, Cat. #60186)  plus 500 μg/ml of Hygromycin B (Hyclone, #SV30070.01) and 5 μg/ml of Puromycin (Invivogen, #ant-pr1).

Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Screen for activators or inhibitors of TIGIT signaling in a cellular context, screen CD155 antibodies for binding affinity, and characterize the biological activity of TIGIT interactions with CD155

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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