CD40/NF-κB Luciferase Reporter HEK293 Cell Line

Catalog #
60626
$6,825 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant HEK293 cell line expressing the full length human CD40 (also known as Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by flow cytometry and western blot. An NF-ĸB luciferase reporter construct is stably integrated into the genome. Thus, the firefly luciferase gene is controlled by 4 copies of an NF-kB response element located upstream of a TATA promoter. Following activation by a CD40 agonist, NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. Activation of CD40 is quantified by measuring luciferase activity.

Illustration of CD40/NF-κB Luciferase Reporter HEK293 Cell Line

Interested in screening and profiling inhibitors, blocking antibodies, or activators of CD40 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line, nfkb, nf-kb
Product Info
Storage and Usage
Citations2
Host Cell Line
HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Supplied As
Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1A BPS Bioscience #79528

 

Materials Used in the Cellular Assay

Name Ordering Information
human TNFα R&D Systems #210-TA
CD40L BPS Bioscience #71191
CD40L antibody BioLegend #310827
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
White clear-bottom 96-well cell culture plate  
Luminometer  
UniProt #
P29965
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

CD40, a member of the TNF receptor superfamily, is expressed in B lymphocytes, antigen presenting cells (APCs) such as monocytes, basophils, and dendritic cells, and in non-immune cells such as endothelial and epithelial cells. The protein is overexpressed in several types of carcinomas. Interaction of CD40 with CD40 ligands (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling increases the activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T cell immunity. CD40 is considered a potential target for cancer immunotherapy.

References

1. Li G et.al. (2013) Human Genetics in Rheumatoid Arthritis Guides a High-Throughput Drug Screen of the CD40 Signaling Pathway. PLoS Genet. 9: e1003487.

2. Pontrelli P et.al. (2006) CD40L Proinflammatory and Profibrotic Effects on Proximal Tubular Epithelial Cells: Role of NF-κB and Lyn. J. Amer. Soc. Neph. 17: 627.

3. Lavorgna A et.al. (2014) A Critical Role for IL-17RB Signaling in HTLV-1 Tax-Induced NF-κB Activation and T-Cell Transformation. PLoS Path. 10: e1004418.

4. Moschonas A et.al. (2012) CD40 Stimulates a “Feed-Forward” NF-ĸB- Driven Molecular Pathway That Regulates IFN-β Expression in Carcinoma Cells. J. Immunol. 188: 5521.

Application References: 

Intein mediated high throughput screening for bispecific antibodies