BRD2 (BD1), GST-tag

Catalog #: 31021
Size: 100 µg
Species: Human
Host Species: E. coli
MW: 41.4 kDa
Genbank #: NM_005104
Tag(s): N-terminal GST-tag
a.a: 65-187
Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 – 187 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 41.4 kDa, expressed in an E. coli expression system.
BRD2, RING, RNF3, Bromodomain containing 2
Assay Conditions:

BRD2 is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen®(R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.
2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance