Biotinylation

 

Biotinylation, Biotin-labeling is commonly used for non-radioactive labeling and purification of proteins and other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) and either avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions of a protein and ligand, exhibiting a dissociation constant (Kd) around 10-15. Furthermore, this interaction is resistant to high or low pH, high salt concentrations, temperature extremes, and denaturants. These properties of the biotin-avidin interaction make it useful for purifying and/or detecting proteins of interest. BPS has a number of proteins that have been biotin-labeled via chemical methods through various reaction groups as well as enzymatically using AviTagTM technology.

In addition to our biotinylated proteins, we also offer a number of reagents that can be used to meet your specific biotinylation needs. There are a number of things to consider when selecting the appropriate biotinylation reagent:

Solubility – Modifications can be made to the biotinylation reagent to increase the solubility, thereby allowing access to hydrophobic or hydrophilic regions within the target protein. In addition, these modifications also increase the solubility of the resulting biotinylated protein. The most common method used to increase solubility of the biotinylation reagent is to add a poly(ethylene glycol) (PEG) spacer arm. The solubility of the biotinylation reagent increases with the number of PEG groups that have been added. BPS offers a variety of PEGylated biotinylation-reagents to meet all of your needs.

Spacer Arm Length – The ability of biotin to bind to avidin/streptavidin is dependent on the availability of biotin.   The spacer arm length is defined as the distance between the conjugated amino acid and the biotin molecule. This length is varied by adding or removing chemical structures in between biotin and the reaction moiety. Increasing this length can increase the availability of biotin for binding to avidin/streptavidin.

Cleavable/Reversible Biotin Reagents – This refers to the ability of the biotin label to be either cleaved, or removed, from the protein or to reverse the biotin-avidin/streptavidin interaction. The former is done by adding spacer arms that include di-sulfide bonds. These disulfide bonds can easily be cleaved under denaturing conditions. The latter is accomplished using modified forms of biotin or avidin/streptavidin that exhibit a lower binding affinity.

Reactive Moiety/Enzymatic Addition – The reactive moiety within a biotinylation reagent determines the specific functional group or amino acid within a protein that will be labeled. Different reactive moieties are available to label primary amines, sulfhydryls, carboxyls, and carbonyls. Additionally, biotin can be enzymatically attached to a protein of interest by using AviTagTM technology. Here, a specific sequence, or AviTagTM, is added to the protein of interest. When this protein is co-expressed with the biotin ligase, BirA, or incubated with BirA under the appropriate conditions in vitro, biotin is site-specifically added to the AviTagTM.

BPS’ portfolio of biotinylation products includes reagents for labeling primary and secondary amines, alkyne groups, thiols, carboxyl groups, and phosphate groups with various numbers of PEG groups. This flexibility allows researchers to meet all of their biotinylation needs. 

Biotinylation Subcategories

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Catalog#SizeProduct NamePrice
72308 50 μg CD48, Fc Fusion (IgG1), Biotin-Labeled (Human) HiP™
$435
71269 50 µg CD58, Fc fusion, Biotin-labeled HiP™
$435
100228 50 µg CD70 His-Avi-Tag, Biotin-Labeled
$420
79127 50 µg CD73, Avi-His-Tag, Biotin Labeled
$435
79416 50 μg CD73, Avi-His-Tag, Biotin-Labeled (Mouse)
$435
100224 100 μg CD83, Avi-His-Tag HiP™
$310
100225 50 μg CD83, Avi-His-Tag, Biotin-Labeled HiP™
$430
71280 50 μg CD96, Fc-Fusion, Avi-Tag, Biotin Labeled (Human)
$435
70206 100 μg CEACAM, Avi-His-Tag
$330
70201 50 μg CEACAM, Avi-His-Tag, Biotin Labeled
$435
40687 50 μg CRTAM, Fc-Fusion (IgG1), Avi-Tag, Biotin-Labeled
$435
79343 50 μg CSF1R, Fc-Fusion, Avi-Tag, Biotin-Labeled
$415
71152

50 µg
500 µg

CTLA4 (CD152), Fc fusion, Biotin-labeled (Human)
Starting at:
$435
79001 50 µg CTLA4 (Mouse), Fc-Fusion (Human), Avi-Tag, Biotin Labeled
$435
72009 96 reactions CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit
$1,070
72024 96 reactions CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit
$1,075
27461 10 x 1L* E. coli in vivo Biotinylation Kit
$530
71286 50 μg FcRn (FCGRT/B2M), His-Avi-Tag, Biotin-Labeled (Mouse)
$435
71283 50 μg FcRn (FCGRT/B2M), His-Tag, Biotin-Labeled, (Human) HiP™
$435
71256 50 ug GITR (CD357), Fc Fusion, Biotin-labeled (Human)
$435
72061 96 reactions GITRL:GITR[Biotinylated] Inhibitor Screening Assay Kit
$1,070
100072 50 µg GPC3, Avi-His-Tagged, Biotin-Labeled
$435
52036 0.5 mg Histone H2a, Full Length, Biotin-labeled, His-tag
$465
52049 50 µg Histone H2a, Full Length, Biotin-labeled, His-tag
$75
52011 80 nmole Histone H3 peptide (1-21), Biotin-labeled
$230

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