aRAF, GST-Tag, His-Tag Recombinant

Catalog #

40010

$385 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human serine/threonine kinase ARAF, encompassing amino acids 282-606 (end). This protein contains mutations Y301D and Y302D. The construct contains a GST-tag at the N-terminal end as well as a C-terminal His-tag (6xhis). The protein was affinity purified.

This product has been cited 1 time.

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Synonyms

ARAF1, RAFA1, aRAF, A-Raf proto-oncogene serine/threonine kinase, Proto-oncogene Pks, PKS, PKS2, A-RAF, ARAF1, RAFA1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Construct

aRAF (GST-282-606(end)-His)

Mutation

YY301, 302DD

Host Species/Expression System

Sf9 insect cells

Purity

≥61%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.

63 kDa

Amino Acids

282–606 (end)

Specific Activity

6 pmol/min/µg.

Genbank #

NM_001654

UniProt #

P10398

Tag(s)

N-terminal GST-tag, C-terminal His-tag

References

1. Yokoyama,T. et al. J. Cell Biol. 177 (5), 781- 793 (2007).
2. Ewing,R.M. et al. Mol. Syst. Biol. 3, 89 (2007).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the BRAF (V600E) Kinase Assay Kit (BPS Bioscience #48688) with aRAF titrated from 800 ng/rxn – 12.5 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40010 Lot 130221-G

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aRAF, GST-Tag, His-Tag Recombinant

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