XIAP (Bir3), His-tag Recombinant

Catalog #

75001

$350 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human x-linked inhibitor of apoptosis (XIAP) Bir3 domain, also known as IAP-like protein, API3, X-linked IAP, and BIRC4, amino-acids 241 – 356 (GenBank Accession No. NM_001167) with N-terminal His-tag, MW = 14.2 kDa, expressed in an E. coli expression system.

This product has been cited 1 time.

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Synonyms

XIAP, IAP-like protein, API3, X-linked IAP, BIRC4

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol

14.2 kDa

Amino Acids

241–356

Genbank #

NM_001167

UniProt #

P98170

Tag(s)

N-terminal His-tag

References

1. Nikolovska-Coleska, Z., et al., Anal Biochem. 2008 Mar, 1; 74(1):87-98.
2. Verhagen, A.M., et al., Cell Death Differ. 2007 Feb;14(2):348-57
3. Fulda, S., Vucic., D., Nat Rev Drug Discov. 2012 Feb 1;11(2):109-24

Application Reference(s):

1. Investigation of Hot Spot Region in XIAP Inhibitor Binding Site by Fragment Molecular Orbital Method (2019)

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in binding and inhibition studies of Caspase 9, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only