XIAP, Bir2-Bir3 Domains, His-Tag Recombinant

Catalog #

75002

$350 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant human XIAP (x-linked inhibitor of apoptosis protein), encompassing amino acids 120-356, corresponding to the Bir2-Bir3 (baculovirus IAP repeat) domains. This construct contains an N-terminal His-tag (6xHis). The protein was affinity purified.

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Synonyms

XIAP, IAP-like protein, API3, X-linked IAP, BIRC4, X-linked inhibitor of apoptosis protein, IAP-like protein, ILP1, MIHA, RING-type E3 ubiquitin transferase XIAP, XLP2, Inhibitor of apoptosis protein 3, IAP-3, hIAP3, hIAP-3, Baculoviral IAP repeat-contain

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

XIAP Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

XIAP (Bir2, Bir3) (His-120-356)

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable Imidazole, 20% glycerol, 3 mM DTT

28 kDa

Amino Acids

120–356

Genbank #

NM_001167

UniProt #

P98170

Tag(s)

N-terminal His-tag

Background

The XIAP (X-linked inhibitor of apoptosis protein), also known as IAP3 (inhibitor of apoptosis protein 3) and BIRC4 (baculoviral IAP repeat-containing protein 4), is a member of the IAP family of proteins. XIAP directly regulates caspases and suppresses apoptotic cell death pathways. XIAP has three Bir (baculovirus IAP repeat) domains, involved in binding to caspases. Bir2 inhibits caspase 3 and 7, while Bir3 is involved in caspase 9 inhibition. An increased expression level of XIAP has been observed in many cancer types, such as prostate cancer. Dysfunction of XIAP has been linked to inflammatory bowel disease, cancer, neurodegenerative and autoimmunity disorders.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The assay was performed in 50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, with 0.5 nM FAM-SMAC peptide (Anaspec #SA-64758), incubated 30 minutes at room temperature. Fluorescence polarization was measured at λexc/λem= 475 nm/528 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only