AMPK (A1/B1/G1), His-tags Recombinant

Catalog #

40025

$615 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. The gene accession number for the three subunits (A1/B1/G1) is NM_006251, NM_006253, and NM_002733, respectively. This protein has been activated with CAMKK.

This product has been cited 1 time.

●

Synonyms

Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.25 mM DTT, 25% glycerol.

~146 kDa (complex)

Amino Acids

full length

Specific Activity

≥300 pmole/min/µg

Genbank #

NM_006251(A1)
NM_006253(B1)
NM_002733 (G1)

UniProt #

A1: Q13131; B1: Q9Y478; G1: P54619

Tag(s)

C-terminal His-tags

Background

AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).

References

1. Minokoshi, Y. et al. Nature 428: 569-574, 2004.
2. Hardie, D G. et al. Eur J Biochem. 1997 Jun 1,246(2):259-73.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using AMARA peptide as a substrate (0.1 mg/ml), 20 μM ATP and 100 μM AMP . Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40025 Lot 190613-1.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

AMPK (A1/B1/G1), His-tags Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY