ALK , GST-tag Recombinant

Catalog #

40214

$355 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human anaplastic lymphoma receptor tyrosine kinase (ALK), also known as CD246 and NBLST3, (GenBank Accession No. NM_004304), a.a. 1058-1620 (end) with N-terminal GST-tag, MW = 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

●

Synonyms

ALK, CD246, anaplastic lymphoma receptor tyrosine kinase, NBLST3

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥60%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol.

89 kDa

Amino Acids

1058–1620 (end)

Specific Activity

77 pmol/min/µg.

Genbank #

NM_004304

UniProt #

Q9UM73

Tag(s)

N-terminal GST-tag

References

1. Ren, H., et al., Cancer Res. 2012,72(13):3312-3323.
2. Zhu, S., et al., Cancer Cell 2012,21(3):362-373.
3. Schonherr, C., et al., Crit Rev Oncog. 2012,17(2):123-143

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was done in a Kinase buffer using 0.2 mg/ml IGF1Rtide as a substrate with 20 µM ATP at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40214_Lot120919-GC2.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

ALK , GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY