AKT3, His-tag Recombinant

Catalog #

40012

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system.

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Synonyms

AKT3, PKBγ, PKBgamma, RACgamma, AKT

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

AKT3 Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

60 kDa

Amino Acids

2–end

Specific Activity

14 pmol/min/µg

Genbank #

NM_005465

UniProt #

Q9Y243

Tag(s)

N-terminal His-tag

References

1. Coffer, PJ. et al. Biochem J. 1998 Oct 1; 335 (Pt 1):1-13.
2. Anderson, KE. et al. Curr Biol. 1998 Jun 4;8(12): 684-91.

Storage/Stability

>6 months at -80°C.

Assay Conditions

The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40012_Lot90212.pdf

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