p300 (KAT3B), His-GST-tag Recombinant

Catalog #

50071

$455 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant human EP300 (E1A binding protein p300), encompassing amino acids 965-1810. The construct contains an N-terminal His-tag (6xHis) followed by a GST-tag. The protein was affinity purified.

This product has been cited 5 times.

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Synonyms

EP300, KAT3B, RSTS2, E1A binding protein, cbp, p300, KAT3B, MKHK2, RSTS2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

P300 Activity

P300 inhibition curve using a chemiluminescent assay (#79705). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations5

Species

Human

Construct

p300 (His-GST-965-1810)

Host Species/Expression System

Sf9

Purity

66%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and variable Imidazole

125 kDa

Amino Acids

965–1810

Genbank #

NM_001429

UniProt #

Q09472

Tag(s)

N-terminal His-GST-tag

References

1. Gui, Y., et al., Nat. Genet. 2011 Aug 7; 43(9) : 875 – 878.
2. Morin, R.D., et al., Nature. 2011 Jul 27; 476(7360) : 298 – 303.
3. Zhao, Z., et al., Brain Res. Bull. 2011 Oct 10; 86(3-4) : 189 – 194.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was performed according to the P300 Chemiluminescent Assay Kit (BPS Bioscience #79705) with p300 titrated from 1.6 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Acetyltransferase

Regulatory Status

Research Use Only