Demethylase & Methyltransferase
     Screening Promo

BPS is now accepting orders and compounds for our March demethylase and methyltransferase screening and profiling promotion. Contact us today for a quote and receive a minimum 10% discount off standard prices and reference inhibitor is included free of charge on orders of all sizes. Pick and choose any or all of our 50+ isozymes. Customers under existing contracts are not eligible. detailed report is included with each order. Submit compounds by March 1st to recieve your report on or before March31st. 

Our team of experts along with our broad services portfolio makes it easy to:

      • Screen for inhibitors/targets using our panel of 19 demethylase assays
        and 32 methyltransferase assays
      • Select from IC50 determination and single point concentrations
      • Receive data within days of compound submission
      • Perform follow-up studies using the same proteins manufactured in-house
      • Get questions answered or project guidance in a time-efficient manner

DNA Demethylase Biochemical Assays:

TET1 TET2

Histone Demethylase Biochemical Assays:

FBXL10 (KDM2B) JHDM1D (KDM7A) JMJD2C (KDM4C) PHF8 (KDM7B)
FBXL11 (KDM2A) JMJD1A (KDM3A) JMJD2D (KDM4D) UTX (KDM6A)
JARID1A (KDM5A) JMJD1B (KDM3B) JMJD2E (KDM4DL)  
JARID1B (KDM5B) JMJD2A (KDM4A) JMJD3 (KDM6B)  
JARID1C (KDM5C) JMJD2B (KDM4B) LSD1 (KDM1A)  

Histone Demethylase Biochemical Assay Format: 

Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above. 

DNA Methyltransferase Biochemical Assays:

DNMT1 DNMT3A/3AL DNMT3B/DNMT3L


Histone Methyltransferase Biochemical Assays:

DOT1L    PRMT9
EZH1 GLP SETDB1
EZH2 MLL1 Complex  SETD2
EZH2 (A677G) NSD1 SET7/9
EZH2-EED NSD2 SET8
EZH2 (Y641C) PRMT1 SMYD2
EZH2 (Y641F) PRMT3 SMYD3
EZH2 (Y641H) PRMT4 SUV39H1
EZH2 (Y641N) PRMT5 SUV39H2
EZH2 (Y641S) PRMT6  SUV4-20H1

Histone Methyltransferase Biochemical Assay Format:

Our histone methyltransferase assays utilize highly specific antibodies that recognize methyl groups. First, SAM is incubated with a sample containing assay buffer and purified recombinant methyltransferase enzyme. Next, acceptor beads, a primary antibody, and then donor beads are added. The final step is to read the Alpha-counts. Note: Image not drawn to scale. 

Contact us today for more information about our Screening and Profiling Services. Tailor our services to meet your needs: Learn more about our Custom Services.

Not quite what you’re looking for? Our Custom Assay Development Services allow for flexibility to meet your specific research needs.