XIAP, FLAG-Tag Recombinant

Catalog #

80401

$415 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant Human XIAP (X-linked inhibitor of apoptosis), full length encompassing amino acids 2-497(end). This construct contains an N terminal flag tag. The recombinant protein was affinity purified.

This product has been cited 2 times.

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Synonyms

API3, BIRC4, IAP3, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP-like protein, ILP, hILP, Inhibitor of apoptosis protein 3, IAP-3, hIAP-3, hIAP3, RING-type E3 ubiquitin transferase XIAP, X-linked IAP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

XIAP Activity

XIAP inhibition curve using a TR-FRET assay (#78306). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

XIAP (FLAG-2-497(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and 80 µg/ml FLAG peptide

57 kDa

Amino Acids

2-497(end)

Genbank #

NM_001167

UniProt #

P98170

Tag(s)

N-terminal FLAG-tag

References

1. Gu L. et al., Cancer Cell 15 (5), 363-375 (2009).
2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to XIAP intrachain TR-FRET Assay Kit (BPS Bioscience #78306) with XIAP titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

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Datasheets/Protocols

80401 Lot 220630-3

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XIAP, FLAG-Tag Recombinant

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