TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway)

Catalog #
60501
$2,505 *
Size: 2 vials
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Description

The TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse and human Wnt3a and to the treatment with an inhibitor of Wnt/ß-catenin signaling pathway.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of the Wnt/ß-catenin signaling pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 18 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
WNT reporter, TCF cell line, LEF cell line, luciferase reporter, TCF/LEF Reporter
Product Info
Storage and Usage
Citations18
Host Cell Line
HEK293 cells
Supplied As
Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Materials Required But Not Supplied

Materials Required for Cell Culture

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1B BPS Bioscience #79531

 

Materials Required for Cellular Assay

Name Ordering Information
LiCl Sigma # L7026
Mouse Wnt3a or human Wnt3a R&D Systems 1324-WN or R&D Systems 5036-WN
IWR-1-endo: inhibitor of Wnt pathway Santa Cruz Biotechnology #sc-295215
Assay Medium: Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1B BPS Bioscience #79531
96-well tissue culture treated white clear-bottom assay plate Corning # 3610
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  
Formulation

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin 

Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)

UniProt #
Q9UJU2 
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Background

The TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) is designed for monitoring the activity of the Wnt/ß-catenin signaling pathway. The Wnt pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of ß-catenin. ß-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.

References

1. Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.