TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway)
The TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse and human Wnt3a and to the treatment with an inhibitor of Wnt/ß-catenin signaling pathway.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of the Wnt/ß-catenin signaling pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
This product has been cited 18 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
Name | Ordering Information |
Thaw Medium 1 | BPS Bioscience #60187 |
Growth Medium 1B | BPS Bioscience #79531 |
Materials Required for Cellular Assay
Name | Ordering Information |
LiCl | Sigma # L7026 |
Mouse Wnt3a or human Wnt3a | R&D Systems 1324-WN or R&D Systems 5036-WN |
IWR-1-endo: inhibitor of Wnt pathway | Santa Cruz Biotechnology #sc-295215 |
Assay Medium: Thaw Medium 1 | BPS Bioscience #60187 |
Growth Medium 1B | BPS Bioscience #79531 |
96-well tissue culture treated white clear-bottom assay plate | Corning # 3610 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
Luminometer |
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin
Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)
The TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) is designed for monitoring the activity of the Wnt/ß-catenin signaling pathway. The Wnt pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of ß-catenin. ß-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.
1. Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.