VEGFR2 (KDR), GST-Tag Recombinant

Catalog #

40301

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human VEGFR2 (Vascular endothelial growth factor receptor 2) also known as Kinase insert domain receptor (KDR), encompassing amino acids 805-1356. This construct contains an N-terminal GST tag. The recombinant protein was affinity purified.

This product has been cited 17 times.

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Synonyms

KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

VEGFR2 Activity

Product Info

Storage and Usage

Citations17

Species

Human

Construct

VEGFR2 (KDR) (GST-805-1356(end))

Host Species/Expression System

Sf9

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT

88 kDa

Amino Acids

805–1356(end)

Specific Activity

≥400 pmol/min/μg

Unit Definition

One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C.

Genbank #

AF035121

UniProt #

P35968

Tag(s)

N-terminal GST-tag

References

1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40301 Lot 191028-GAB

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VEGFR2 (KDR), GST-Tag Recombinant

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