VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line

Catalog #

79387

$12,205 *

Size: 2 Vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line is a HEK293 cell line expressing the firefly luciferase reporter under the control of NFAT response elements. The cells also stably express human VEGFR2 (Human Vascular Endothelial Growth Factor Receptor 2; KDR, FLK1; ref seq. NM_002253.1).

Expression of VEGFR2 was assessed by flow cytometry and this cell line has been functionally validated with Sorafenib, Anti-VEGF Neutralizing Antibody and Aflibercept Biosimilar.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of VEGFR2 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 17 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1, NFAT, VEGF

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Product Data Gallery

Expression of VEGFR2 in VEGFR2/NFAT HEK293 Recombinant Cell Line by flow cytometry

Dose response curve of VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line to VEGF165

Dose response curve of VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line to Sorafenib

Dose response curve of VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line to Anti-VEGF Neutralizing Antibody

Dose response of VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line to Aflibercept

Product Info

Storage and Usage

Citations17

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1A	BPS Bioscience #79528

Materials Used in the Cellular Assays

Name	Ordering Information
Human VEGF165	BPS Bioscience #91001
Thaw Medium 1	BPS Bioscience #60187
Protease-free Bovine Serum Albumin (BSA)	Sigma-Aldrich #A4919
Sorafenib	LC Laboratories #S-8599
Anti-VEGF Neutralizing Antibody	BPS Bioscience #79478
Aflibercept Biosimilar	Ichorbio #ICH4015
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
Luminometer

UniProt #

P35968

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The interaction between VEGF (Vascular Endothelial Growth Factor) and its receptor VEGFR is an important mediator of angiogenesis as well as endothelial cell survival and proliferation. VEGFR2 is a receptor tyrosine kinase that plays a major role in cellular responses driven by VEGF. Targeting the kinase activity of VEGFR2 has been an attractive tool in cancer therapy based on its anti-angiogenesis effects. In addition to the kinase inhibitors, humanized monoclonal antibodies interfering with VEGF/VEGFR2 interaction have been also developed for anti-cancer treatments.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1A (BPS Bioscience #79528): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin and 100 μg/ml of Hygromycin B

Media Required for Functional Cellular Assay

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Assay Medium: Prepare the Assay Medium using DMEM medium supplemented with 1% (w/vol) protease free BSA (Sigma-Aldrich #A4919)

Applications

Screen for activators or inhibitors of VEGF/VEGFR2 signaling pathway
Screen for inhibitors of VEGFR2 kinase activity

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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VEGFR2/NFAT Reporter HEK293 Recombinant Cell Line

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