UTX (KDM6A), N-terminal FLAG-tag Recombinant

Catalog #

50119

$475 *

Size: 20 µg

Qty

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Description

Human UTX, also known as KDM6A (GenBank Accession No. NM_021140) (amino-acids 1-1401) with N-terminal FLAG-tag, MW = 155 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

UTX, KDM6A

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

UTX (KDM6A) inhibition curve using an AlphaLISA assay (#50516). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Baculovirus-infected Sf9 cell

Purity

≥58%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 80 μg/ml flag peptide and 0.2mM TCEP

155 kDa

Amino Acids

1-1401

Specific Activity

0.096 pmol/min/μg

Genbank #

NM_021140

UniProt #

O15550

Tag(s)

N-terminal FLAG-tag

References

1. Greenfield A, et al. (1998). Hum. Mol. Genet. 7 (4): 737–42.
2. Agger K, et al. (2007). Nature 449 (7163): 731–4.

Application Reference(s):
1. Inhibition of KDM4A activity as a strategy to suppress interleukin-6 production and attenuate colitis induction (2017)
2. Identification of the KDM2/7 histone lysine demethylase subfamily inhibitor and its antiproliferative activity (2013)

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary.

Assay Conditions

10 µl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1mM TCEP, 100 µM alpha-ketoglutarate, 25 µM iron, 0.1mM ascorbic acid, 0.02% Tween-20, 0.5 µM biotinylated peptide substrate, and UTX (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Demethylase

Regulatory Status

Research Use Only