USP8, FLAG-tag

Catalog #: 80358
Size: 50 µg
$415
Species: Human
Host Species: Sf9 insect cells
MW: 128 kDa
Genbank #: NM_005154
Tag(s): N-terminal FLAG-tag
a.a: 2–1118(end)
Description:
Human USP8 (Ubiquitin-Specific Protease 8) or UBPY, GenBank Accession No. NM_005154, a.a. 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
UniProt
P40818
Synonym(s):
USP 8, Ubiquitin-Specific Protease 8, UBPY, USP-8
Specific Activity:
6.5 pmole/min/µg
Assay Conditions:
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Naviglio, S., et al. (1998). EMBO J. 17(12):3241-50.
2. Niendorf, S., et al. (2007). Mol Cell Biol. 727(13):5029-39.
3. Mizuno, E., et al. (2005). Mol. Biol. Cell. 16(11): 5163-74.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitins: Deubiquitinase (DUB)
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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