USP7, His-FLAG-Tags Recombinant

Catalog #

80395

$425 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human USP7 (ubiquitin specific peptidase 7), full length, encompassing amino acids 2-1102(end). This construct contains an N-terminal His tag (6xHis) fused with a Flag-tag. The recombinant protein was affinity purified.

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Synonyms

Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

USP7 inhibition curve using a fluorogenic assay (#79256). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

USP7 (His-FLAG-2-1102(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable mM imidazole and 20% glycerol

130 kDa

Amino Acids

2–1102 (end)

Specific Activity

545 pmole/min/µg

Genbank #

NM_003470

UniProt #

Q93009

Tag(s)

N-terminal His-FLAG-tags

References

1. Fernandez-Montalvan, A., et al., FEBS J. 2007 Aug;274(16):4256-70.
2. Li, M., et al., Mol Cell. 2004 Mar 26;13(6):879-86.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

This Assay was performed according to USP7 Inhibitor Screening Assay Kit (BPS Bioscience #79256) with USP7 titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80395 Lot 220720-1

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USP7, His-FLAG-Tags Recombinant

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