USP2, FLAG-Tag (Sf9-derived) Recombinant

Catalog #

80352

$440 *

Size: 50 µg

Qty

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Description

Recombinant human USP2 (ubiquitin specific peptidase 2), full length, encompassing amino acids 2-605(end). This construct contains an N-terminal Flag-tag. The recombinant protein was affinity purified.

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Synonyms

USP 2, Ubiquitin-Specific Protease 2, UPB41, USP-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

USP2 inhibition curve using a fluorogenic assay (#78859). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

USP2 (Sf9-derived) (FLAG-2-605(end))

Host Species/Expression System

Sf9

Purity

≥55%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable FLAG peptide

69 kDa

Amino Acids

2-605(end)

Specific Activity

2.9 pmol/min/µg

Genbank #

NM_004205

UniProt #

O75604

Tag(s)

N-terminal FLAG-tag

Background

Ubiquitin specific peptidase 2 (USP2) belongs to a large group of ubiquitin-specific proteases capable of cleaving ubiquitin from other proteins. These enzymes are also referred to as deubiquitinating peptidases, deubiquitinases (DUBs), ubiquitin proteases, ubiquitin hydrolases or ubiquitin isopeptidases. They contribute to the ubiquitin signaling pathway by countering the signal induced by ubiquitin conjugating enzymes and ligases. DUBs are new therapeutic targets for neurodegenerative diseases, cancer, diabetes, and autoimmune pathologies.

References

1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.
2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-32.
3. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to USP7 Inhibitor Screening Assay Kit (BPS Bioscience #79256) with USP2 (BPS Bioscience #80352) titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80352 Lot 221212-S

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USP2, FLAG-Tag (Sf9-derived) Recombinant

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