USP2, His-tag (E. coli-derived) Recombinant

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Catalog #

80392

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Description

Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 259-605(end) with an N-terminal His-tag, MW=40.9 kDa, expressed in an E. coli cell expression system.

This product has been cited 1 time.

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Synonyms

USP 2, Ubiquitin-Specific Protease 2, UPB41, USP-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

E. coli

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 15 mM ?-mercaptoethanol, 250 mM imidazole, and 20% glycerol

40.9 kDa

Amino Acids

259–605(end)

Specific Activity

247 pmol/min/µg

Genbank #

NM_004205

UniProt #

O75604

Tag(s)

N-terminal His-tag

References

1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.
2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-32.
3. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.

Application Reference(s):

1. Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway (2020)

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80392_Lot120928.pdf

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