USP15, SUMO-His-tags Recombinant

Catalog #

81100

$360 *

Size: 25 µg

Qty

Bulk/Custom

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Description

Human USP15 recombinant protein, with N-terminal SUMO- and His-tags, expressed in E. coli.

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Synonyms

Deubiquitinating enzyme 15, KIAA0529, MGC131982, MGC74854, Ubiquitin carboxyl-terminal hydrolase 15, Ubiquitin-specific-processing protease 15, Ubiquitin thioesterase 15, Unph-2, Unph-4, UNPH4, USP 15, USP-15

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Format

Aqueous buffer solution

Formulation

50 mM Na phosphate pH 7.5, 50 mM NaCl, 1 mM DTT

121 kDa

UniProt #

Q9Y4E8

Tag(s)

N-terminal SUMO-His-tags

Background

USP15 is a deubiquitylase that associates with the COP9 signalosome (CSN) complex. It is able to regulate the NF-kappaB pathway by deubquitylating IkappaBalpha2.

References

1. Schweitzer K., et al., CSN controls NF-κB by deubiquitinylation of IκBα. EMBO J., 26(6):1532-41 (2007).
2. Hetfeld, BK., et al., The zinc finger of the CSN-associated deubiquitinating enzyme USP15 is essential to rescue the E3 ligase Rbx1. Curr Biol, 15(13):1217-21 (2005).

Storage/Stability

Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.

Assay Conditions

The activity of USP15 and USP2core are measured with 100 nM DiUb48 in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, 0.02% Tween-20

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

This enzyme is active in the Ub-CHOP assay.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deubiquitinase

Regulatory Status

Research Use Only

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USP15, SUMO-His-tags Recombinant

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