USP14, FLAG-tag Recombinant

Catalog #

80364

$425 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), GenBank Accession No. NM_005151, a.a. 2-494(end) with an N-terminal FLAG-tag, MW=57 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

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Synonyms

USP 14, Ubiquitin-Specific Protease 14, TGT, tRNA-guanine transglycosylase, USP-14

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

USP14 inhibition curve using a fluorogenic assay (#78865). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

57 kDa

Amino Acids

2–494(end)

Specific Activity

5.7 pmole/min/µg

Genbank #

NM_005151

UniProt #

P54578

Tag(s)

N-terminal FLAG-tag

References

1. Chen, P.C., et al. (2009). J. Neurosci. 29(35):10909-19.
2. Chen, Y.C., et al. (2010). RNA 16(5):958-68.
3. Nagai, A., et al. (2009). Biochem. Biophys. Res. Commun. 379(4):995-1000.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80364__lot120227_R.pdf

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USP14, FLAG-tag Recombinant

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