Recombinant human UHRF1 (ubiquitin like with PHD and ring finger domains 1), full length, encompassing amino acids 2-793(end). This construct contains an N terminal His tag (6xHis) followed by a Flag-tag. The recombinant protein was affinity purified.
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Synonyms
UHRF1, Ubiquitin-Like With PHD And Ring Finger Domains, RING Finger Protein 106, RNF106, Np95, ICBP90
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, and 100 µg/ml FLAG peptide
MW
92 kDa
Amino Acids
2-793(end)
Genbank #
NM_001048201
UniProt #
Q96T88
Tag(s)
N-terminal His-FLAG-tags
Background
Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, it also plays a key role in chromatin modification. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. May be involved in DNA repair. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating
topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications.
References
1. Kofunato, Y., et al., Oncol Rep. 2012 Dec; 28(6):1997-2002. 2. Jazirehi, A.R., et al., Epigenomics. 2012 Jun;4(3):251-252. 3. Jenkins, Y., et al., Mol Biol Cell. 2005 Dec;16(12):5621-9.
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.