UEV1A (UBE2V1), FLAG-tag Recombinant

Catalog #

80325

$290 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human UEV1A (ubiquitin conjugating enzyme E2 V1), full length encompassing amino acids 2-170 (end). The protein was constructed with an N-terminal FLAG-Tag. The recombinant protein was affinity purified.

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Synonyms

CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1, TRAF6-regulated IKK activator 1 beta Uev1A, ubiquitin-conjugating enzyme E2 variant 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

UEV1A (FLAG-2-170(end))

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3mM DTT, 100 µg/ml FLAG peptide.

20 kDa

Amino Acids

2–170(end)

Genbank #

NM_021988

UniProt #

Q13404

Tag(s)

N-terminal FLAG-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Reaction was performed according to the UBCH13 TR-FRET Assay Kit (BPS Bioscience #79741) with various amounts of UEV1A.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Conjugating Enzyme (E2)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80325 Lot 210908

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UEV1A (UBE2V1), FLAG-tag Recombinant

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