UCHL3, His-tag Recombinant

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Catalog #

80353

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Description

UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002), a.a. 2-231(end), with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.

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Synonyms

UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3, Ubiquitin C-terminal Hydrolase L3

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

UCHL3 inhibition curve using a fluorogenic assay (#78815). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

27.1 kDa

Amino Acids

2–231(end)

Specific Activity

≥20,000 pmole/min/µg

Genbank #

NM_006002

UniProt #

P15374

Tag(s)

N-terminal His-tag

References

1. Wada, H., et al. (1998). Biochem. Biophys.
     Res. Commun. 251 (3): 688–92.
2. Nam, M.J., et al. (2004). Proteomics 3 (11):
     2108–15.
3. Setsuie, R., et al. (2009). Neurochem Int.
     54(5-6):314-21.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL3 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Hydrolase

Regulatory Status

Research Use Only

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Datasheets/Protocols

80353_Lot100826.pdf

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UCHL3, His-tag Recombinant

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